Lesson 3 – Tools of the Trade Exploratorium: Collecting Oceanographic Data From Where We Cannot See

Description: Students deepen their understanding of Earth’s systems and develop an understanding of how explanations are built from data when they see how scientists use the tools in the field. Six stations will help students learn how oceanographers measure and collect data from where we cannot be or see using both basic and advanced technological tools.

They will apply the information learned in this lesson to piece together that the ocean has layers that form unique ecosystems with chemical and physical variations dependent on the atmospheric conditions and the depth of the layer. Each layer supports microbes with genetic characteristics and variations that allow you to make comparisons. Energy transfer and nutrient cycles support interdependent species and create niches in the ocean ecosystem. Variations in niches lead to differences in genetic makeup.


See the NGSS listed as buttons in the left-hand menu and in the chart below. The buttons on the left are grouped to show the integrated three-dimensional nature of our lessons and modules. When applicable, if NGSS are addressed outside of bundles, they are listed separately. Connections to 21st Century Learning Skills and other published standards are also included in the chart below. In addition, for this lesson, here is a breakdown of:

What students learn
  • How measurements are collected at the scale of both surface and deep ocean
  • Technical tools are available to collect data from the simple cultured in the lab-scale to the larger global scale
  • Data can be used to compare environmental conditions that influence homeostasis in ocean phytoplankton and other populations
What students do
  • Explore and define the different tools for measuring and collecting evidence about ocean microorganisms, and the ocean ecosystem (small and large scales)
  • Use models of BLAST bioinformatics and flow cytometry to explore ocean phytoplankton diversity
Aligned Washington State Standards (Next Generation Science Standards)
Performance expectation(s): HS-LS4-1: Communicate scientific information that common ancestry and biological evolution are supported by multiple lines of empirical evidence; HS-PS4-5: Communicate technical information about how some technological devices use the principles of wave behavior and wave interactions with matter to transmit and capture information and energy

The bundle of performance expectations above focuses on the following elements from the K-12 Science Education Framework:

Highlighted Science and Engineering Practice(s)

Highlighted Disciplinary Core Idea(s)

Highlighted Crosscutting Concept(s)

SEP-1: Asking questions and defining problems

SEP-3: Planning and Carrying out an Investigation*

SEP-8: Obtaining, Evaluating, and Communicating Information

LS4.A: Evidence of Common Ancestry and Diversity

LS1.A: Structure and Function

PS3.D: Energy in chemical processes and everyday life

PS4.A: Wave Properties

PS4.C: Information Technologies and Instrumentation

CCC-1: Patterns

CCC-2: Cause and Effect

CCC-4: Systems and System models

CCC-7: Stability and Change

Common Core: M1, M2, E1, E2, E5, E6

CTE: Depending on level of interaction with the tools, this lesson has many potential applications to learning 21st Century skills.

STEM application, skills building, engineering design, problem solving

*Depending on level of interaction with the tools and opportunity to design, this Practice can be met when combined with Lesson 4, where data collection and analysis is fulfilled. Working in teams it can build communication and collaboration, information media/technology and life and career skills. The connections to content in the module helps build environmental literacy, engineering design, problem-solving, and global awareness.



How can you measure the things you cannot see? If land vegetation dies off how will our oxygen be generated? Is it enough to continue to support life on Earth? It is challenging to “see” both very small, and very big things—from the tiny Prochlorococcus introduced in Lesson 2, to all ocean phytoplankton that produce fifty percent of the world’s oxygen. The “Exploratorium” introduces the world of discovery of oceanographers’ Anne Thompson and Sally Chisholm. The six hands-on stations explore the current technological tools that oceanographers, biologists and physical ocean scientists use to study the dynamic ocean and living organisms. The Exploratorium shows how they can measure what “we cannot see” at global scales. Students deepen their understanding of Earth’s systems and develop understanding of how explanations are built from data when they see how scientists use the tools in the field. It is a good way to introduce genetic diversity, evolution, or a deeper study of genetically diverse microbes. After this lesson, use Lab 3.5 to experience the phenomenon of phytoplankton fluorescence as a tool for measuring chlorophyll distribution. Later, in Lesson 4 and the more advanced Lesson 5, apply knowledge of these tools through data-driven activities that exemplify oceanographic research of the Invisible Forest.

All the tools introduced in the “Exploratorium” are referred to throughout the Module.  PLEASE NOTE – we are field testing both in-person and virtual versions of this lesson.  Please refer to this spreadsheet (or use this link – https://tinyurl.com/u6ylcqm) for a compilation of all links and updates.  We will also update this lesson webpage and information as materials are finalized.


Plan time: Optimizing time and combining Lab 3.5 with Stations

Table 1 – Overview of Instructional Activities:

Activity What students learn What students do
Part 1: Introduction Activity (~10 min) [Field test options] Use NOVA Video: “Earth from Space” (~10 min.) and/or Activity 1-3_ Tools of the Trade — How will we monitor and protect the ocean?, (+10 min.) with (optional) Explorer Question CARDS and Explorer Questions_sorting organizer. – What and where we measure matters

-There are different types of data measuring tools in oceanography

– Make observations of ocean phytoplankton, recognizing differences in scale.

– Compare ocean research questions and the scale data is collected and measured

Part 2: Stations Activity (~30-40 min) Tour the classroom Tools of the trade: Exploratorium stations with Tools of the trade_Exploratorium_Stations Organizer (in PDF)

(Teachers see: Tools of the Trade HOW TO SET UP; here is the Tools of the Trade Organizer KEY)

– How measurements are collected at the scale of both surface and deep ocean

– Technical tools are available to collect data from the small cultured in the lab scale to the larger global scale.

– Data can be used to compare environmental conditions that influence homeostasis in ocean phytoplankton and other populations

– Explore and define the different tools for measuring and collecting evidence about ocean microorganisms, and the ocean ecosystem (small and large scales)

– Use models of BLAST bioinformatics and flow cytometry to explore ocean phytoplankton diversity

Part 3: Assessment (~20-30 min) How will I know they know…

Use Tools of the Trade: Story of Discovery about Sally “Penny” Chisholm [winner of the 2019 Crafoord science prize] [Field test option] End with Video: Annenberg Learning —Unit 3: Oceans//Section 1: (min.13-15)
(+2 min)

Or use PBL reflections ‘Know /Need to know’ and PBL summary table to add to ‘Drop of Seawater’ art projects

– What do I know now?

– Tools can be applied and combined to develop deeper understanding of phytoplankton populations and the oxygen they produce

– [PBL option] Making connections and seeing relationships in Drop of Seawater. What questions do I still need/want to ask?

– Fill in the blanks to demonstrate understanding of how to apply the tools in a real life story.

– Discuss ‘big picture’ questions as a class.

– [PBL option] Conduct reflection and ask new questions by adding to ‘Drop of Seawater’ art project.


Part 1: Introduction (~5-10 min.)

Take students from the very small microscopic scale to the very large macroscale and ask them to think how to measure and answer questions about the deep ocean.


Step 1: Introduce the Essential Question(s) using NOVA Video: “Earth from Space” (show 1h13m to 1h23m) OR the first three slides of: Tools of the trade: Exploratorium stations powerpoint. Follow up the teacher-led discussion in the powerpoint with: Activity 1-3_ Tools of the Trade — How will we monitor and protect the ocean? Ask them to Pair-share, then write in lab journals.

Activity 1 (1 min): Ask What does the ocean do for us? Observe and compare [slides 1 and 2] the two images [at different scales] in the powerpoint and the two samples of water containing tiny aquatic organisms (mostly diatoms). As learned in Lesson 2, phytoplankton abundance and scale is varied, we are going to study them in macro-scale as well as nano-scale. One kind of ocean data is ‘Observational’ data, as you see in these slides.

Activity 2 (1 min): Ask Does it matter what we measure? Students are introduced to the questions of measuring at both very large and very small scale by briefly sorting the Explorer Question CARDS using Explorer questions_sorting organizer.

Activity 3 (3 min): Does it matter where we measure? As students observe the two satellite images (‘remote sensing’) it gives them a first glimpse of the macro scale and global observation. Ask What other ocean data is available? Write a list of their ideas on the board. Ask as formative assessment: Do satellite images provide measurable data? quantity? Stop here. [The remaining slides in the powerpoint are printed out to be displayed as information at each station of the “Tools of the Trade: Exploratorium”.]


Part 2: Tour of the Tools Exploratorium (Stations Activity) (40-60 min)

Introduction: Tell them they are about to enter the “Tools of the Trade: Exploratorium” a self-guided experience—a mini science center.

Step 1: Divide students into groups of 2-3 and start each group at one of the 6 stations to begin their exploration. Along the way they can discover how oceanographic data collection is possible with current technology.

Step 2: Ask them to think about this Focus question: How are we going to protect, manage, and monitor changes in the ocean system? What evidence would you collect to answer your investigation questions [either their own PBL questions or ‘Explorer questions’]? We can collect cells living in a drop of seawater. How do we know how many and what kind of cells they are? Give them a little background for how they will apply their learning later:

The tiniest Prochlorococcus, was discovered only in the last 30 years, because new technology allows oceanographer Sally “Penny” Chisholm PhD [winner of the 2019 Crafoord science prize] to “see” the cells that are 5000 times smaller than a chloroplast. Others like Anne Thompson, PhD continue to ask new questions and make discoveries. What tools do oceanographers use to learn about these cells on the larger global scale and the very small scale to help answer research questions?

Step 3: Hand out Tools of the trade_Exploratorium_Stations_Organizer (PDF) worksheet or record in lab journals. Student reminder: How do we measure in science? [Take a moment to remind students that when using calibrated tools, scientists measure to the last unit of certainty and estimate to the next decimal place. UNITS of what you measure (“grams of salt”) matter!]

Step 4: Working in groups, visit each of the 6 stations. Allow 5-10 min per station (stations vary in complexity). Encourage independent exploration, while circulating to help develop thinking (examples of teacher questioning strategies are in station preparation notes).

Step 5: Students with extra “downtime” may work on writing descriptions into their “Stations Organizer” worksheet or lab journal. Which of their investigation questions can they begin to answer? Do they need to collect more evidence?* (*If finished early or concurrently, students may begin preparing phytoplankton samples to use in Lab 3.5 Phytoplankton Spectrophotometry and Fluorescence for more in-depth, hands-on learning.)


Part 3: Review and Apply Exploratorium Tools (10 min)

Introduction: Read about Sally “Penny” Chisholms’ [winner of the 2019 Crafoord science prize] Tools of the Trade—Story of Discovery to learn about her work and apply learning from this lesson:

Step 1: Hand out (blank) version of Tools of the Trade—Story of Discovery. Students work in groups of two to read the story using the descriptions to help ‘Fill in the blanks’ with the name of the tool that she used for each new discovery in the story.


Step 2: As students read ask them to take notes, to build understanding of how the various tools were “bundled” (used in combinations) over time and what they contributed to making the discoveries about Synechococcus* and the huge final Discovery of the very small Prochlorococcus(?) (*single-cell cyanobacteria).


Step 3: Conduct a student self-check for understanding by reviewing the story. Ask for students’ help to fill in the answers on the overhead (formative assessment).


Step 4: Discuss these questions with the whole class [pair-share first, from ideas they compiled on the back of their sheet]:

  • What part of the scientific question did each tool help to answer? [Remember this happened over many years, and many expeditions.]
    1. What were the big questions “The Story of Discovery” investigations helped to answer?


Step 5: End with Video: Annenberg Learning —Unit 3: Oceans // Section 1: Introduction (show minutes 13- 15) (2 min) —an introduction to scientist Penny Chisholm in her lab talking about Prochlorococcus

Step 6: [PBL Option] Review learning (10 min) Use PBL reflections ‘Know /Need to know’ and the PBL summary table to help formulate ideas, before adding new components to their ‘Drop of Seawater’ art.

Final Step: Go on to Lab 3.5 Phytoplankton Spectrophotometry and Fluorescence


How will I know they know……

  • Observe the work students do in the application of this lesson– how well are they able to sort through the tools and apply tools with reasoning. Look at their notes in the Organizer:
    • Does it matter where we take these measurements? Did students use a justification for where they want to take the samples (through Layers? Over time? Along a transect?)
    • Have they shown that they are thinking about scale, and what is possible if we collect a lot of data from a lot of drops of ocean water?
    • What question(s) will each tool help us answer and what data can we get using it? (ex. “Data collected from CTD could be useful for…” )
  • Look at what students have added to the drop of seawater using the Drop of seawater Summary Table (Google Doc | Word Doc). How have they shown the relationships between the tools and how they can help scientists understand the systems found in seawater and oxygen production?

Use student responses to Tools of the Trade: Story of Discovery the “Penny Chisholm story of discovery.” Are they understanding how scientists think about the data they are collecting? Are they demonstrating understanding of how similarities in different cells collected in the ocean can apply to wider populations — if so how?


  • Teacher Resource – Tools of the Trade: Story of Discovery (Key) (Google Doc | Word Doc)

Annenberg Learning: Video Unit 3: Oceans // Section 1: Introduction (start at 13 min.)


  • The stations lead into more in depth studies in Lab 3.5: Fluorescing & Spec (link here to lab) and Lesson 4 and 5, where this understanding is applied in activities using current ocean data.
  • BLAST activity: Computer based (bandwidth limited if attempted with entire class)
  • Design a bundle of tools to send down on a cast from an oceanography expedition, out of ALOHA. Good lead into HOT DOGS datasets in Lesson 5. (Link here to design worksheet)
  • Planktonplanet.org – series of images of collecting data, students would send samples, or download data from this site. Claims, listed – what evidence did they show?
  • http://globecarboncycle.unh.edu/cmapTP.shtml# To show students how carbon is calculated (need permission to use one of the lessons in here about calculating carbon footprint/tree as carbon sink)
  • Calculating oxygen content at depths: http://ocean.ices.dk/Tools/Calculator.aspx

Here are additional ideas for tools to learn about and discuss with students. What can be measured—that is outside of the tools already presented? How would they add to the story?

  • Fe isotope measuring (amount of oxidized Fe in rock or shells?)
  • Magnetism of earth’s crust (growth of earth’s crust over geologic time)
  • Cell counts and culturing cells in controlled lab environment
  • Photosynthesizers in lab environment – rate of oxygen production, then expand to the number of types of cells in sample area of ocean



Animations and videos: How tools work. (Listed here)

Short videos demonstrating different ocean physical characteristics that can be measured. http://www.divediscover.whoi.edu/circulation/demonstration.html


  1. Canfield, Donald E. “Oxygen: A Four Billion Year History,” Science Essentials, Dec 1, 2015
  2. Dutkiewicz, et al, Understanding Microbial Competition for Nitrogen, The Darwin project, MIT News (2018),
  3. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263930/
  4. https://www.oceanfdn.org/calculator
  5. http://www.livescience.com/27551-our-amazing-planet-top-to-bottom-mountaintop-to-ocean-trench-infographic.html
  6. http://www.divediscover.whoi.edu/tools/index.html
  7. http://www.divediscover.whoi.edu/tools/seanet.html
  8. http://www.divediscover.whoi.edu/tools/sonar.html
  9. The Scale of the Microbial World, Marion Field Fass. Microbes Count!
  10. https://socratic.org/questions/how-do-you-calculate-molality-from-molarity
  11. https://www.aqua-calc.com/page/density-table/substance/seawater
  12. Oceanic observations of the Pacific 1950
  13. A.F. Post, The Prokaryotes: Vol. 4: Bacteria: Firmicutes, Cyanobacteria, pp. 1104, Chpt 2.3, (2006) Springer, edited by S. Falkow, E. Rosenberg, K.-H. Schleifer, E. Stackebrandt
  14. J. P. Zehr & Bess B. Ward, Nitrogen Cycling in the Ocean: New Perspectives on Processes and Paradigms (2002) DOI: 10.1128/AEM.68.3.1015-1024.2002
  15. S. Rabouille, et al, Modelling the vertical distribution of Prochlorococcus and Synechococcus in the North Pacific Subtropical Ocean, Microbrial Ecology, 17 July 2007; https://doi.org/10.1111/j.1462-2920.2007.01376.x
  16. Rex R Malmstrom, […] S. Chisholm, et al. Temporal dynamics of Prochlorococcusecotypes in the Atlantic and Pacific oceans, The ISME Journal, vol. 4, pgs. 1252–1264, (May 2010)

Mikhail V. Zubkov, et al, High Rate of Uptake of Organic Nitrogen Compounds by Prochlorococcus Cyanobacteria as a Key to Their Dominance in Oligotrophic Oceanic Waters, Microbial Ecology, Feb 2003, 69 (2) 1299-1304; DOI: 10.1128/AEM.69.2.1299-1304.2003

Ways of seeing Experimentation: